- Whole body mouse imaging in cross-sections
- Imaging of endogenous chromogens (e.g. hemoglobin, oxygenated hemoglobin, melanin, lipid,…)
- Imaging of exogenous chromogens (e.g. near-infrared fluorescent dyes, gold nanoparticles, nanortubes,…)
- High resolution in depth (± 100 µm)
- Real time imaging
Optoacoustic (also called photoacoustic) in vivo imaging is a novel imaging technic that is at the junction of fluorescence imaging and ultrasound imaging. A pulsed laser-light causes a thermoelastic expansion that generates ultrasounds, which are then recorded to reconstruct the image. Optoacoustic imaging combines advantages of both worlds because it combines the molecular specificity and the high sensitivity of fluorescence with the high spatial and temporal resolutions of ultrasound imaging. Moreover, it offers a greater penetration depth than classical optical modalities because ultrasounds are far less absorbed by biological tissues than photons.
MultiSpectral Optoacoustic Tomography (MSOT) scans the mouse (full body) in successive cross-sections that can be combined to reconstruct a 3D volume. Moreover, it allows simultaneous discrimination of multiple chromogens. Indeed, endogenous chromogens, such as hemoglobin, oxygenated-hemoglobin, melanin and lipid, are visible in real-time, simultaneously, distinctly, and without any injection into the mouse. Furthermore, exogenous chromogens, such as near-infrared (NIR) fluorescent dyes, gold nanoparticles, nanorods…, can be injected in order to record specific anatomic, functional or molecular events.
- Mouse full body imaging
- Cross-sectional images at high spatial resolution (± 100 µm) that allow 3D reconstruction
- Anatomical information
- Functional information: perfusion, oxygenation
- Molecular information using probes as NIR fluorescent dyes, gold nanoparticles, etc.
- Ideal to study oxygenation in cancer topics
- Tracking of cells expressing NIR fluorescent proteins
- Real-time imaging, so ideal for pharmacokinetics and DCE studies
- Simultaneous imaging of multiples chromogens
- Integrates systems to anesthetize (isoflurane), warm and monitor the animal.
- MSOT InVision 256-TF (iThera Medical)
Photoacoustic temperature imaging based on multi-wavelength excitation.
Meng L, Deschaume O, Larbanoix L, Fron E, Bartic C, Laurent S, Van der Auweraer M, Glorieux C
Photoacoustics 2019 Mar; 13: 33-45
|Facility manager||Academic supervisor|
Photo credits: Lionel Larbanoix