Holographic microscopy

Description and applications

Digital holographic microscopy allows to record the information of an entire experimental volume as a hologram. The hologram can be treated to refocus the sample at different depths without temporal distortion. It also allows to carry out the interferometric measure of optical thickness with nanometric precision (quantitative phase contrast)

This optical microscopy technique has applications in numerous research fi elds such as monitoring of cells in culture, precise measurements inside cells (i.e. refractive index), the observation of particles in a fl ux and 3D tomography.

The laboratory of Physical Chemistry of the Université libre de Bruxelles (ULB) has developed an original holographic microscopy technology. That is covered by a number of patents. It generates images with a quality similar to that of classical microscopes in different modes such as bright fi eld, DIC and quantitative phase contrast with a high capacity of in depth investigation, as well as the combination with fl uorescence.

The digital holographic microscopy has been applical to many areas of research monitoring of cell cultures, cell fusion, migration of tumour cells in thick collagen gels as well as velocimetry and the analysis of particles in a fl uid. A patented microscope has also been used in the International Space Station (ISS) for the observation of protein crystallization in microgravity.

Equipment

 

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Holographic microscopy