Microscopy

Electron microscopy

  • Transmission electron microscope (TEM)
  • Ultrastructural analysis
  • Immunolocalization
  • Scanning electron microscope (SEM)
  • Surface morphology analysis
  • Forthcoming developments:
  • Cryo-TEM
  • structural analysis of samples in suspension
  • TEM-assisted tomography
  • 3D reconstruction of nanoparticles and cellular sections

Automation and quantitative microscopy

Digital holographic microscopy

  • Cell counting and evaluation of cell viability for adherent cells or cells in suspension
  • Counting of lipid droplets in adipocytes

Fluorescence microscopy

  • Wide-field
  • Laser-scanning confocal (CLSM)
  • Spinning-disk confocal (SD)
  • High-throughput microscope
  • Localization of molecules of interest at diverse scales
  • 3D reconstruction
  • live imaging (including of yeast and bacteria)
  • With the CLSM: spectral imaging, FRAP, FRET

Contact:

Academic supervisor Academic supervisor

Benoît Vanhollebeke
Benoit.Vanhollebeke@ulb.ac.be

David PEREZ-MORGA
David.Perez-Morga@ulb.ac.be

Photo credits: François de Ribaucourt, C. Lamy