Digital pathology

DIAPath

DIAPath (Digital Image Analysis in Pathology) is a platform oriented towards the validation of tissue-based biomarkers using standardized immunohistochemistry (IHC) and slide digitalization processes. It proposes services that range from sample preparation to image and data analysis:

  • Cell and tissue processing (fixation, paraffin embedding)
  • Tissue microarray production
  • Slide sectioning and automated staining (H&E, PAS, Masson trichrome, …)
  • Standardized immunohistochemistry (IHC), sequential IHC, chromogenic in situ hybridization (CISH) (Complementary information)
  • Whole slide high-resolution imaging
  • Qualitative and semi-quantitative scoring by experts (double blind test)
  • Quantitative image analysis (morphometry, staining characterization, …) (Complementary information)
  • Tissue-based biomarker colocalization (Complementary information)
  • Data analysis and reporting

Applications

  1. Customized services related to pathology, histology and IHC
  2. Imaging and digital pathology: macro/microscopy imaging, whole slide scanning, customized image analysis
  3. Screening: drug target, protein expression
  4. Validation: animal model, biomarker, antibody, companion diagnostics

Equipment

  1. Automated immunohistochemistry system (Ventana Discovery XT)
  2. Automated tissue micro-arrayer (Alphelys MiniCore)
  3. Slide scanner (Hamamatsu Nanozoomer C96000-01)

Description

DIAPath is a multidisciplinary and inter-faculty unit (ULB Schools of Medicine and of Engineering) which offers an integrated solution to histological analysis as well as the identification, characterization and validation of tissue-based biomarkers. The methodology is based on standardized laboratory procedures and quality controls ensuring reproducibility and traceability. It involves the following technologies : (i) macroscopic analysis (organs, tissues, etc.), (ii) microscopic histological analysis (using haematoxilin-eosin and special staining), (iii) tissue microarray (TMA) and cell-block (for cell line analysis), (iv) immunohistochemistry (IHC), (v) whole slide imaging and image analysis, (vi) statistical data analysis. IHC has the advantage to preserve tissue morphology and thus antigen location at histological and cell levels. By simultaneously processing thousands of samples, the TMA technology allows standardized screening of protein expression using IHC and thus provides a very efficient way for biomarker validation. Slide scanning and image analysis enable archiving, sharing and quantitative staining characterization. Finally, data analysis enables biomarkers to be statistically validated and compared.

Representative publications:

Contact:

Facility manager Academic supervisors
optique003
Cédric BALSAT
Cedric.BALSAT@ulb.ac.be
diapath@cmmi.be
optique003
Christine DECAESTEKER
cdecaes@ulb.ac.be
optique003
Isabelle SALMON
isabelle.salmon@erasme.ulb.ac.be

 

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