Pr Isabelle Salmon, responsable (Erasme)
isabelle.salmon@erasme.ulb.ac.be
Isabelle Salmon obtained a MD degree in 1985 and was certified specialist in Pathology in 1991. She obtained an “Agrégation de l'Enseignement Superieur” degree (Ph.D. degree and qualification for university professorship) in Medical Sciences in 1993 from the Université Libre de Bruxelles (ULB). Since 1999, she is the head of the Pathology Department of the ULB Erasme Hospital which manages the diagnostic activity for all the hospital services including polyclinics. She teaches pathology at the ULB School of Medicine. Her research interests include glioma biology and the identification of biomarkers in the context of different non-tumoral and tumoral pathologies, with a particular focus on neuropathology. She published more than 250 articles in peer reviewed journals. She is SBAP-BVAP and BWP chairperson and is member of numerous scientific (national and international) societies. Together with Christine Decaestecker she created DIAPATH, an interfaculty research center (Faculty of Applied Sciences and School of Medicine) from ULB. DIAPATH’s activities focus on the identification of diagnostic, prognostic and theragnostic biomarkers as well as on the analysis and characterization of animal models, using a multidisciplinary and integrated approach based on histology, immunohistochemistry, image and data analysis.
Pr Christine Decaestecker, responsable (ULB, LISA)
cdeceas@ulb.ac.be
Christine Decaestecker obtained a PhD degree in Pure Science (Mathematics) in 1991 and an “Agrégation de l'Enseignement Superieur” degree (qualification for university professorship) in 1997 from the Université Libre de Bruxelles (ULB). She is Senior Research Associate with the “Fonds National de la Recherche Scientifique” (Belgian national funds for scientific research, Brussels, Belgium). She is a member of the Laboratory of Image Analysis and Synthesis (LISA) from the ULB Faculty of Applied Sciences and teaches data analysis and machine learning. She also is the chairperson of the biomedical engineering degree at the ULB Faculty of Applied Sciences. Her research interests include machine learning, pattern recognition and image analysis applied to biomedical fields (more particularly cell biology and clinical research). She has more than 150 publications in peer reviewed journals. Together with Isabelle Salmon she created DIAPATH, an interfaculty research center (Faculty of Applied Sciences and School of Medicine) from ULB. DIAPATH’s activities focus on the identification of diagnostic, prognostic and theragnostic biomarkers as well as on the analysis and characterization of animal models, using a multidisciplinary and integrated approach based on histology, immunohistochemistry, image and data analysis.
Sauvage Sébastien, M. Sc.
sebastien.sauvage@erasme.ulb.ac.be
Born in October, 1976 in Haine-Saint-Paul (Belgium), Sébastien Sauvage obtained his Master degree in chemistry in 2000 at the University of Mons, Belgium. He obtained his “D.E.A.” in molecular biology in 2002 at the same university. His “D.E.A.” was focused on the Fascioscapulohumeral dystrophy (FSHD). He studied the proteins profile by 2D SDS-PAGE of this disease and specially the expression of two proteins members of the DUX family (DUX4 and DUX4c) that are involved in the mechanism of this muscular dystrophy. After three years in Unibioscreen (oncology discovery & development specialized company) and two years in the Pathology Department of the ULB Erasme Hospital, he works now in the DIAPATH unit within The Center for Microscopy and Molecular Imaging (CMMI). He works with Lionel Larbanoix on the development of immuno-histochemistry protocols and on the identification, characterization and validation of protein biomarkers.
Paul Barbot was born in 1985. He studied Computer Science and Software Engineering at EPITA in Paris from which he graduated with an Engineering title. He then moved to the Netherlands to work on CT/MR 3D visualisation software at Philips Healthcare. He is now working at the CMMI on image processing of pathology images.
Angélique FRANCOIS
angelique.francois@ulb.ac.be
Angélique FRANCOIS was born in 1974. She obtained her baccalaureate degree in clinical chemistry in 1996 from the HEPH Condorcet Charleroi.
She realized different pharmacokinetic analysis by HPLC for SGS Biopharma (Wavre).
During 10 years she worked for UCB in Braine l'Alleud and she acquired technical expertise in pathology and immunohistology focused on CNS desease
Following a restructuring, she worked for two years at the institute of pathology and genetic (IPG Gosselies) as a technician histology and immunochemistry R&D.
Before joining the DIAPATH unit, she worked for one year in the department of pathology of the Erasme Hospital. Her activities were focused on the application of basic histological technique and on the development of immuno-histochemistry protocols and on the identification, characterization and validation of protein biomarker.
DIAPATH
Équipements
- Scanner Hamamatsu NanoZoomer acquis par le CMMI (visualisation d’un spot de TMA)
- Automate d’immunohistochimie VENTANA - Discovery XT

Références bibliographiques
- Mathieu A, Weynand B, Verbeken E, Da Silva S, Decaestecker C, Salmon I, Demetter P. Comparison of four antibodies for immunohistochemical evaluation of epidermal growth factor receptor expression in non-small cell lung cancer. Lung Cancer 2010;69:46-50. IF = 3.1.
- Rorive S, D'Haene N, Fossion C, Delpierre I, Abarguia N, Avni F, Decaestecker C, Salmon I. Ultrasound-guided fine-needle aspiration of thyroid nodules : stratification of malignancy risk using follicular proliferation grading, clinical and ultrasonographic features. Eur J Endocrinol 2010;162:1107-15. IF=3.5.
- Laurendeau I, Ferrer M, Garrido D, D'haene N, Ciavarelli P, Basso A, Vidaud M, Bieche I, Salmon I, Szijan I. Gene expression profiling of the Hedgehog signaling pathway in human meningiomas. Mol Med. 2010. Jul-Aug;16(7-8):262-70.
- Flamez D, Roland I, Berton A, Kutlu B, Dufrane D, Beckers MC, De Waele E, Rooman I, Bouwens L, Clark A, Lonneux M, Jamar JF, Goldman S, Maréchal D, Goodman N, Gianello P, Van Huffel C, Salmon I, Eizirik DL. A genomic-based approach identifies FXYD domain containing ion transport regulator 2 (FXYD2) gamma as a pancreatic beta cell-specific biomarker. Diabetologia 2010;53:1372-83. IF = 6.5.
- Pozdzik AA, Berton A, Schmeiser HH, Missoum W, Decaestecker C, Salmon IJ, Vanherweghem JL, Nortier JL. Aristolochic acid nephropathy revisited: a place for innate and adaptive immunity? Histopathology 2010;56:449-63. IF = 3.8.
- Rivas MN, Hazzan M, Weatherly K, Gaudray F, Salmon I, Braun MY. NK cell regulation of CD4 T cell-mediated graft-versus-host disease. J Immunol 2010;184:6790-8. IF = 5.6.
- Maréchal R, Mackey JR, Lai R, Demetter P, Peeters M, Polus M, Cass CE, Salmon I, Devière J, Van Laethem JL. Deoxycitidine kinase is associated with prolonged survival after adjuvant gemcitabine for resected pancreatic adenocarcinoma. Cancer 2010;15:116(22):5200-6.
- Maréchal R, De Schutter J, Nagy N, Demetter P, Lemmers A, Devière J, Salmon I, Tejpar S, Van Laethem JL. Putative contribution of CD56 positive cells in cetuximab treatment efficacy in first-line metastatic colorectal cancer patients. BMC Cancer 2010;10:340.
- Rorive S, Moles-Lopez X, Maris C, Trepant A-L, Sauvage S, Sadeghi N, Roland I, Decaestecker C, Salmon I. TIMP-4 and CD63: New Prognostic Biomarkers in Human Astrocytomas. Mod Pathol. 2010 Oct;23(10):1418-28.
- Decaestecker C, et al. Requirements for the valid quantification of immunostains on tissue microarray materials using image analysis. Proteomics 2009;9:4478-94.
- D’Haene N, et al.. Endothelial hyperplasia and endothelial galectin-3 expression are prognostic factors in primary central nervous system lymphomas. Br J Haematol 2008;140:402-10.
- D’Haene N, et al. The differential expression of Galectin-1 and Galectin-3 in normal lymphoid tissue and non-Hodgkin’s and Hodgkin’s lymphomas. Int J Immunopathol Pharmacol 2005;18:431-43.
- Mathieu A, et al. Development of a chemoresistant orthotopic human nonsmall cell lung carcinoma model in nude mice: analyses of tumor heterogenity in relation to the immunohistochemical levels of expression of cyclooxygenase-2, ornithine decarboxylase, lung-related resistance protein, prostaglandin E synthetase, and glutathione-S-transferase-alpha (GST)-alpha, GST-mu, and GST-pi. Cancer 2004;101:1908-18
Descriptif et applications
DIAPATH est un centre transdisciplinaire et interfacultaire qui offre une solution intégrée pour l’analyse histologique ainsi que l’identification, la caractérisation et la validation de biomarqueurs protéiques. Cette solution est basée sur des procédures standardisées et des contrôles de qualité assurant reproductibilité et traçabilité. Elle intègre les technologies suivantes : (I) l’analyse macroscopique, (II) l’analyse histologique microscopique (hématoxyline-éosine et colorations spéciales), (III) le tissue microarray (TMA) et le cell-block (pour lignées cellulaires), (IV) l’immunohistochimie (IHC), (V) le scanning de lames histologiques et l’analyse d’image, (VI) l’analyse statistique des données.
L’IHC a l’avantage de préserver l’aspect morphologique du tissu analysé, et ainsi la localisation de l’antigène ciblé au niveau histologique et cellulaire. Par le traitement simultané de milliers d’échantillons, la technologie de TMA apporte une standardisation accrue de l’IHC et offre un outil de choix pour la validation de biomarqueurs.
Le scanning de lames et l’analyse d’image permettent l’archivage ainsi qu’une caractérisation objective et quantitative des immunomarquages. Enfin, l’analyse de données offre une validation et une comparaison statistique des biomarqueurs ainsi caractérisés.
http://www.ulb.ac.be/rech/inventaire/unites/ULB723.html
Voir le poster: Poster_CMMI_-_Diapath_-_compressed.pdf



