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    • Microscopie électronique
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Pr Robert Muller, Directeur scientifique CMMI et responsable (Umons)
robert.muller@umons.ac.be

Robert N. Muller was born in 1948 and studied at the University of Mons-Hainaut (UMH), Belgium, from which he was graduated with a Lic. Sci. Degree in chemistry in 1969. He obtained his PhD in 1974 from the same University where he was successively appointed Assistant, Lecturer and full Professor. He is currently head of the Department of General, Organic and Biomedical Chemistry and, since October 2005, Dean of the Faculty of Medicine and Pharmacy of the University of Mons. He did post-doctoral studies in Magnetic Resonance Imaging in Paul C. Lauterbur’s* research group at the State University of New York at Stony Brook in 1981-82, collaborated with Drs Seymour Koenig in the domain of fast field cycling relaxometry and was on a sabbatical leave at the Center for Magnetic Resonance (CERM), Florence, Italy with Professors Ivano Bertini and Claudio Luchinat in 2002-2003. Co-founder of the European Workshop on Nuclear Magnetic Resonance in Medicine; Vice-Chairman of the European Magnetic Resonance Forum Foundation, 1991-present; President of the European Society for Magnetic Resonance in Medicine and Biology, 1987-1988; President of the GRAMM (Groupe de Recherche sur les Applications du Magnétisme en Médecine), 1998-2000; Vice-Chairman of the COST D18 Action, 2003; Founding member of the European Society for Molecular Imaging (ESMI); Editor in chief of the new journal Contrast Media and Molecular Imaging (Wiley) and Member of the editorial boards of Magnetic Resonance Materials MAGMA (Springer) and Investigative Radiology (Lipincott). He has produced around 200 publications and contributed to 6 books mainly in NMR relaxometry, spectroscopy and imaging in the context of the development and applications of contrast agents for Molecular Imaging. Relevant awards: Outstanding teacher award of the International Society for Magnetic Resonance in Medicine ISMRM (2005) Outstanding teacher award of the International Society for Magnetic Resonance in Medicine ISMRM (2007) Contrast Media Research Award (2009) Fellow of the European Society for magnetic Resonance in Medicine and Biology (2010) *2003 Nobel Prize in Medicine or Physiology

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Sébastien Boutry, Dr Sc, En charge des manipulations d'IRM et d'imagerie optique / in vivo (Umons)
sebastien.boutry@umh.ac.be

Sébastien Boutry was born in 1978 and studied at the University of Mons (UMons) from which he graduated with a M.Sc. Degree (Biology) in 2001. He became a researcher in the nuclear magnetic resonance (NMR) and molecular imaging laboratory of Prof. Robert N. Muller (UMons). Using the magnetic resonance imaging (MRI) technique as a tool for biological studies, he first focused on MRI contrast agents (gadolinium-based as well as iron oxide-based) specific for molecular markers of inflammation, developed in the laboratory (six papers were published). He obtained his Ph.D. in Biology in 2007. During his thesis, he performed and published several studies on the magnetic labelling of cells with iron oxide-based nanoparticular MRI contrast agents. He also attempted to use specifically vectorized nanoparticles as tools for the tracing of brain neurons in the context of an Interuniversity Attraction Poles program. This specific cellular targeting aspect was also approached in a work he started as a “FNRS – Télévie” scientific collaborator in 2006, aiming at the development of a specifically targeted contrast agent which might allow for the MRI monitoring of the efficiency of pro-apoptotic drugs used for anti-cancer therapies. In 2008, he became a research collaborator in the Center for Microscopy and Molecular Imaging (CMMI). He is developing biological applications of NMR (MRI and MR spectroscopy), but also other techniques of molecular imaging such as optical imaging and nuclear medicine.

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Mathieu Roch, Bach Elect. (Umons)
mathieu.roch@umons.ac.be

Mathieu Roch was born in 1983. He studied at the “Roi Baudoin” High School (Mons) from which he graduated with a B.Sc. Degree (Electronics) in 2004. For 4 years, he worked in the electronic development of traffic radars for the Belgian society SIRIEN. In 2009, he joined the Center for Microscopy and Molecular Imaging (CMMI) as a technical collaborator responsible for the maintenance of NMR equipments and for the running of MRI and optical imaging experiments.

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Lionel LARBANOIX, Dr Sc
lionel.larbanoix@umons.ac.be

Lionel Larbanoix: born in August, 1982 in Uccle (Belgium), he obtained his Master degree in biology in 2005 from the University of Mons, Belgium. He obtained his Ph.D. in Biomedical Sciences in 2010 from the same university. His Ph.D. thesis was focused on the magnetic resonance imaging (MRI) of Alzheimer’s disease. He screened peptide libraries by using the phage display technology in order to find specific ligands of amyloid deposits. These ligands were then grafted on MR contrast agents to allow in vivo visualisation of amyloid plaques (results published in Neurobiology of Aging in 2008). He worked for two years in the DIAPATH unit within The Center for Microscopy and Molecular Imaging (CMMI). His activity was focused on the development of immunohistochemistry protocols and on the identification, characterization and validation of protein biomarkers. He is now a collaborator in the MRI and Optical Imaging units within the CMMI. His activity is focused on the development of biological applications of optical imaging and MRI.

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Liste des axes > Imagerie optique

Imagerie optique

 

Équipements

  1. Le Photon Imager du CMMI (Biospace Lab, France)


Références bibliographiques

  1. A bimodal system (luminophore and paramagnetic contrastophore) derived from Ln(III) complexes based on a bipyridine-containing macrocyclic ligand, I.Nasso, C. Galaup, F. Havas, P. Tisnes, C. Picard, S. Laurent, L. Vander Elst, R.N. Muller, Inorg. Chem., 44(23):8293-8305 (2005)
  2. A dual lanthanide probe suitable for optical (Tb3+ luminescence) and magnetic resonance imaging (Gd3+ relaxometry), N. Geum, I. Nasso, B. Mestre, P. Tisnès, C. Picard, S. Laurent, R.N. Muller, L. Vander Elst, Bioorg.Med.Chem.Lett.,16, 5309-5312 (2006)
  3. In vitro characterization of the Gd complex of [2,6-pyridinediylbis(methylene nitrilo)] tetraacetic acid (PMN-tetraacetic acid) and of its Eu analogue, suitable bimodal contrast agents for MRI and optical imaging, S. Laurent, L. Vander Elst, C. Galaup, R.N. Muller, C. Picard, Bioorg Med Chem Lett., 17(22), 6230-6233 (2007)

Descriptif et applications

La technique d’imagerie optique permet de détecter la fluorescence et la bioluminescence chez de petits animaux (rats, souris).

La bioluminescence naturelle n’existe que chez quelques espèces animales (lucioles, coraux marins) et repose sur une réaction enzymatique. L'oxydation de la luciférine (chez la luciole) ou de la coelenterazine (chez le corail "Renilla" (pensée de mer)) catalysée par leur luciférase respective conduit à la production de lumière. Pour mener des études en bioluminescence sur des cellules de mammifères, il est nécessaire de les modifier génétiquement afin qu’elles produisent de la luciférase. L’imagerie de bioluminescence permet par exemple l’étude de l’invasion tumorale et de traitements antitumoraux ou celle de la migration de cellules souches (voir figure).

Pour les études basées sur la détection de la fluorescence, des traceurs ciblant des molécules ou des régions d’intérêt, par exemple des récepteurs membranaires surexprimés en situation pathologique, sont administrés. La conception de nouveaux traceurs spécifiques est menée en collaboration avec le Service de Chimie Générale, Organique et Biomédicale de l’Université de Mons (http://portail.umons.ac.be/fr/universite/facultes/fmp/services/service1/pages/default.aspx).

Voir la vidéo: http://www.eyecone.com/clients/LabInsight/ULB-IRM-IO/

Voir le poster: POSTER_CMMI_-_Optical_Imaging.pdf

 
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