Pr Oberdan LEO, ULB IBMM, IMI
oleo@ulb.ac.be
Professor Oberdan Leo is Professor at the Université Libre de Bruxelles (ULB), and Assistant Professor at the Université de Mons (UMons) Belgium, where he teaches Immunology. Since 1999, he has served as President of the Belgian Immunological Society. Professor Leo's
major research interests focus on the relationship between metabolism and the inflammatory response and the analysis of T helper subset differentiation pathways. He is presently Director of Institute for Medical Immunology, a research institute dedicated to Clinical and Experimental Immunology created in 2004 thanks to a joint public -private research program initiated by GSK Biologicals, ULB and the Walloon Region.
Frédéric Lhommé, PhD, (ULB, IMI)
flhomme@ulb.ac.be
Frédéric C. Lhommé was born in 1971 and studied at the Ecole Nationale Supérieure de Chimie et Physique de Bordeaux, France from which he was graduated as Engineer in Chemistry and Physics in 1994. He obtained a PhD in Physics with a specialty in optics from the University of Metz, France in 2000. His research was dealing with the study of the effects of the chromium concentration and crystal composition in LiNbO3:Cr3+ by optical spectroscopy. He did post-doctoral studies about the modelling of the light self –focusing in photorefractive materials at Supélec, Metz, France in 2001 and about the inscription and use of Bragg gratings in optical fibres for sensor applications at the Electromagnetism and Telecommunications Group of the Faculté Polytechnique de Mons, Belgium from 2001 to 2005. Since 2006, he is at the head of the Flow Cytometry unit of the Institute of Medical Immunology of the Université Libre de Bruxelles, Gosselies, Belgium. His research interests are mainly in the development of flow cytometry or related technologies and protocols for research applied to the study of inflammation and transplantation. He published around 25 publications.
Nicolas Passon, Technicien en charge des phases exploitation et routine
npasson@ulb.ac.be
Nicolas Passon was born in 1985. He graduated in mathematics and sciences from the high school Saint Joseph at Charleroi. He studied biology during two years at the UMH (University of Mons Hainaut). He obtained a bachelor’s degree in clinical biology in 2008. As a bachelor, he studied the synergy between HDAC inhibitors and Chaetocine in the process of HIV-1 retrotranscription inhibition. He currently works at the CMMI (Center for Microscopic and Molecular Imagery) unit headed by Dr Frédéric Lhommé. He provides technical assistance in informatics, cell culture, molecular biology, flow cytometry, cell sorting and flow cytometry imaging.
Imagerie par cytométrie en flux
Équipements
L’équipement consiste en un cytomètre en flux à résolution morphologique ImageStream 100 (Amnis, Seattle). Il permet de d’acquérir 6 images différentes par cellule et comporte 3 sources laser d’excitation (bleu 488 nm, rouge 658 nm et violet 405 nm) ainsi qu’une source lumineuse blanche.
Figure 1 - ImageStream 100 (Amnis, Seattle)
Figure 2 - Localisation du facteur de transcription NF-κB dans des lymphocytes T avant (A) et après (B) stimulation par anti-CD3. De gauche a droite, les images correspondent respectivement à l’image enregistrée avec une source blanche (BF), NF-kB, le marqueur nucléaire Draq5 et la vue composite de NF- κB et Draq5.
Descriptif et applications
L’imagerie par cytométrie en flux est une technologie qui permet de bénéfi cier à la fois dans un seul et même instrument de la puissance visuelle de la microscopie et de la rigueur statistique associée à la cytométrie en flux. En d’autres mots, elle permet d’acquérir rapidement des images de cellules dans un flux puis de les analyser qualitativement mais également quantitativement selon des critères tels que la morphologie, la localisation ou l’intensité de traceurs sur, dans ou encore entre des cellules.
De nombreuses applications peuvent être citées telles que les études fonctionnelles, phénotypage, cycle cellulaire, mort cellulaire, translocation nucléaire de facteurs de transcription (Figure 2), classifi cation de souspopulations cellulaires … dans un large éventail de disciplines des sciences du vivant (immunologie, microbiologie, parasitologie, vaccinologie, thérapie cellulaire ….).
Voir le poster: POSTER_CMMI_-_Imaging_Flow_Cytometry.pdf
