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Prof. Véronique KRUYS, IBMM ULB
vkruys@ulb.ac.be

Véronique Kruys was born in 1963 and studied at the Université Libre de Bruxelles (ULB), Belgium, from which she graduated with a Lic. Sci. Degree in 1985. She obtained her Ph.D. in 1989 from the same University while she was appointed Assistant. During her thesis, she identified a major mechanism controlling cytokine gene expression at the post-transcriptional level which was reported in papers published in PNAS and Science. She went on post-doc for three years (1990-1002) in the Laboratory of Bruce Beutler, at the Howard Hughes Medical Institute, University of Texas at Dallas. On her return at the Free University of Brussels, she was appointed Assistant Professor at the Department of Molecular Biology of the Faculty of Sciences. Her scientific interest remained focused on biological processes controlling messenger RNA metabolism. She published around 40 publications and several reviews. She has been teaching biochemistry and molecular biology of genes for several years. In the recent years, her research interests focused on the characterization of protein and RNA traffic which led her to develop her expertise in fluorescence microscopy.

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Axes list > Fluorescence Microscopy

Fluorescence Microscopy

 

Equipment

  1. Inverted fluorescence microscope Zeiss Axio Observer Z1, with a motorized stage, temperaturecontrolled incubation chamber equiped with perfusion device. Images are acquired with a high-speed digital camera (Axiocam Hsm) or with a high-resolution Coolsnap HQ camera. Colibri/Lambda DG4 double light source provides excitation for imaging of UV/blue/green fluorochromes.
  2. Inverted confocal microscope Leica TCS SP2 (excitation λ : 458, 476, 488, 514, 543, 633 nm)
  3. Confocal microscope Zeiss LSM 710 NLO (multiphoton ready):
    • Inverted and motorized microscope
    • 7 laser lines : 405 nm, 458 nm, 488 nm, 514 nm, 543 nm, 594 nm and 633 nm
    • Objectives :
    o 10x/0,30 EC Plan-Neofluar WD=5,2 mm DIC ∞/- D=0,17 mm
    o 20x/0.80 Plan-Apochromat WD=0,55 mm DIC ∞/- D=0,17 mm
    o 40x/1.20 C-Apochromat Water DIC Corr D=0,14-0,19 mm
    o 63x/1.40 Plan-Apochromat Oil DIC D=0,17 mm
    • HXP 120C metal halide lamp with several filtersets to image commonly used fluorochromes (DAPI (49), Cy3 (43) and GFP (38), …)
    • For an optimal control of the temperature (live imaging): Incubator XL multi S1 around the microscope and Heating insert P S1 inside to dock culture chambers into the stage. Possibility to control gas composition inside the incubator.
    • QUASAR 34-channels detector : 32-element array PMT detectors and two flanking single PMT detectors, enabling acquisition of the entire spectral range as a λ-stack in one single scan
    • ZEN software with additional modules:
    o Image VisArt plus (3D and 4D animation)
    o ROI HDR (high dynamic range imaging)
    o FRAP
    o FRET

 


Bibliographical references

  1. Pendrin: the Thyrocyte Apical Membrane Iodide Transporter? L. Twyffels, C. Massart, P. E. Golstein, E. Raspe, J. Van Sande, J.E. dumont, R. Beauwens, V. Kruys. Cell Physiol Biochem 2011;28:491-496, Oct. 17,2011
  2. Shuttling SR proteins: more than splicing factors. Twyffels L, Gueydan C, Kruys V.

  3. FEBS J. 2011 Jul 27. doi: 10.1111/j.1742-4658.2011.08274.x.

  4. The HTLV-1 Tax protein inhibits formation of stress granules by interactin with histone deacetylase 6. Legros S, Boxus M, Gatot JS, Van Lint C, Kruys V. Kettmann R, Twizere JC, Dequiedt F. Oncogene. 2011 May 2. 
  5. The splicing factor ASF/SF2 is associated to TIAR/TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression. N. Delestienne, C. Wauquier, R. Soin, J.-F. Dierick, C. Gueydan and V. Kruys (last 2 authors equally contributed). FEBS J. (2010) 277, 2496-2514.
  6. Impaired embryonic development in mice overexpressing the RNA-binding protein TIAR. Y. Kharraz, P.-A. Salmand, A. Camus, J. Auriol, C. Gueydan, V. Kruys, D. Morello. PLoS ONE (2010) 5(6) e11352.
  7. Identification of the sequence determinants mediating the nucleo-cytoplasmic shuttling of TIAR/TIA-1 RNA-binding proteins. T. Zhang, N. Delestienne, G. Huez, V. Kruys, and C. Gueydan. J. Cell Sci. (2005) 118, 5453-5463.
  8. The Vacuole System Is a Significant Intracellular Pathway for Longitudinal Solute Transport in Basidiomycete Fungi. P.R. Darrah, M. Tlalka, A. Ashford, S.C. Watkinson, and M.D. Fricker. Eukaryotic Cell (2006) 5, 1111-1125.
  9. Intracellular NAD levels regulate TNF- protein synthesis in a sirtiun-dependent manner. F. Van Gool, M. Galli, C. Gueydan, V. Kruys, P.-P. Prévot, A. Bedalov, R. Mostoslavsky, F. Alt, T. De Smedt and O. Leo. Nature Medicine (2009) 15, 206-210.
  10. Post-transcriptional regulation of genes encoding anti-microbial peptides in drosophila. A. Lauwers, L. Twyffels, R. Soin, C. Wauquier, V. Kruys and C. Gueydan. J. Biol. Chem. (2009) 284, 8973-8983

Description and applications

  1. 3D and time-lapse imaging and analysis of molecule subcellular distribution by direct fluorescence in living cells or by direct and indirect fluorescence in fixed cells and tissues.
  2. Co-localisation of fluorescent signals.
  3. Imaging and analysis of molecular interactions in living or fixed cells by FRET.
  4. Imaging and analysis of molecular diffusion by FRAP.
  5. Deconvolution of fluorescent signals and 3D-reconstruction.

See the poster: POSTER_CMMI_-_Fluorescence_Microscopy.pdf

See the "Molecular Biology of the Gene" website: http://www.ulb.ac.be/ibmm/homeuk_4.html

 
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