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Associate Prof. David PÉREZ-MORGA, IBMM ULB
david.perez-morga@ulb.ac.be

David Pérez-Morga was born in México City in 1960 and obtained his Bachelor degree in Microbiology at the National School of Biological Sciences from the National Polytechnic Institute (IPN) in México. He got his Ph. D. Degree in Molecular Biology in 1989 on lambda phage biology at the CINVESTAV (IPN). From 1989 to 1993 he performed post-doctoral studies on mitochondrial DNA replication of trypanosomatids at the School of Medicine of the Johns Hopkins University in the USA. His main honours are the Rockefeller Foundation Fellowship (1989), the EMBO Fellowship (2003) and the Prix de l’Académie Royal de Médecine de Belgique (2005). Since 2005 he is an associate professor at the Université Libre de Bruxelles. He has published 29 peer-reviewed scientific papers on high impact factor journals such as Cell, Science, J. Cell Biol., PNAS, EMBO J, and J. Mol. Biol. His main research interests are biology of trypanosomatids and cell biology of the cilium. His expertises are in molecular biology and in optical and electron microscopy.

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Marjorie VERMEERSCH, B. Sc.
mvermee1@ulb.ac.be

Marjorie VERMEERSCH was born in December 1976, She obtained her baccalaureate degree in clinical chemistry in 1999 from the HE Charleroi Europe (Fleurus). She performed her final training in the department of pathology of Erasme Hospital (headed by Isabelle Salmon) where she acquired technical expertise in pathology and immunohistology focused on microglia. She worked for 2 more years in the same department. In 2001, with the relocation of the Université Libre de Bruxelles in Gosselies, she joined the laboratory of Immunology, headed by Jacques Urbain. She worked on dendritic cells under the supervision of Muriel Moser. During the past eight years she acquired technical expertise in immunology, histology, immunohistology and confocal microscopy. In April 2009, she joined the CMMI unit dedicated to the development of electron transmission microscopy, scanning microscopy, ultramicrotomy, and ultracryotomy.

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Axes list > Electron microscopy

Electron microscopy

 

Equipment

  1. Transmission Electron Microscope. Tecnai 10 (FEI)
  2. Environmental Scanning Electron Microscope. Quanta 200 FEG (FEI)
  3. Room temperature-and Cryo-Ultramicrotomes. Leica EM UC6 and UC7
  4. Critical point dryer Leica EM CPD030.
  5. High Vacuum CoatingSystem Leica EM MED020.

Bibliographical references

  1. The cationic lipid, diC14 amidine, extends the adjuvant properties of aluminum
    salts through a TLR-4- and caspase-1-independent mechanism
    Arnaud Wilmar, Caroline Lonez, Marjorie Vermeersch , Mathieu Andriannea , David Pérez-Morgac,d,
    Jean-Marie Ruysschaertb, Michel Vandenbrandenb, Oberdan Leoa, Stéphane T. Temmermana,2011 Elsevier Ltd, 27 october 2011

  2. J Exp Bot. 2011 Jul;62(11):4055-65. Epub 2011 May 4.
    Ntann12 annexin expression is induced by auxin in tobacco roots.
    Baucher M, Oukouomi Lowe Y, Vandeputte OM, Mukoko Bopopi J, Moussawi J, Vermeersch M, Mol A, El Jaziri M, Homblé F, Pérez-Morga

  3. J acoby M, Cox JJ, Gayral S, Hampshire DJ, Ayub M, Blockmans M, Pernot E, Kisseleva MV, Compère P, Schiffmann SN, Gergely F, Riley JH, Pérez-Morga D, Woods CG, Schurmans S (2009). INPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse. Nature Genet. 41(9):1027-31.

  4. Herman M, Pérez-Morga D, Schtickzelle N, Michels PA (2008). Turnover of glycosomes during life-cycle differentiation of Trypanosoma brucei. Autophagy. 4(3):294-308.
  5. Pays E, Vanhollebeke B, Vanhamme L, Paturiaux-Hanocq F, Nolan DP, Pérez-Morga D (2006). The trypanolytic factor of human serum. Nature Rev Microbiol. 4(6):477-86.
  6. Pérez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homblé F, Vanhamme L, Tebabi P, Pays A, Poelvoorde P, Jacquet A, Brasseur R, Pays E (2005). Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes. Science. 309(5733):469-72.
  7. García-Salcedo JA, Pérez-Morga D, Gijón P, Dilbeck V, Pays E, Nolan DP (2004). A differential role for actin during the life cycle of Trypanosoma brucei. EMBO J. 23(4):780-9.
  8. Pérez-Morga DL, Englund PT (1993). The attachment of minicircles to kinetoplast DNA networks during replication. Cell. 74(4):703-11.

Description and applications

A. Transmission A. Electron Microscopy (TEM)

  1. Ultra-structural analysis of ultra-thin sections of tissues and isolated cells.
  2. Particle analysis by negative staining of phages, viruses and other nanoparticles.
  3. DNA and RNA imaging by spreading and shadowing techniques.
  4. R oom temperature- and cryo-ultramicrotomy (cryo-sectioning)
  5. I mmuno-gold localisation on resin-embedded ultra-thin sections, or on cryo-sections by the Tokuyasu method.
  6. Sample preparation for all the above mentioned analyses.

B. Scanning Electron Microscopy (SEM)

  1. Analysis of conductive (metalized) and non conductive (environmental) biological samples
  2. Analysis of nanomaterials
  3. Sample preparation for SEM

C. Qualitative and quantitative image analyses.

D. Consultancy and training in EM.

See the video : http://www.eyecone.com/clients/LabInsight/ULB-IBBM/

See the poster: POSTER_CMMI_-_Electron_microscopy.pdf

 
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