Fluorescence microscopy

Applications

  1. 3D and time-lapse imaging and analysis of molecule subcellular distribution by direct fluorescence in living cells or by direct and indirect fluorescence in fixed cells and tissues.
  2. Co-localisation of fluorescent signals.
  3. Imaging and analysis of molecular interactions in living or fixed cells by FRET.
  4. Imaging and analysis of molecular diffusion by FRAP.
  5. Deconvolution of fluorescent signals and 3D-reconstruction.

Equipment

  1. Laser-scanning confocal microscope Zeiss LSM 710
    Equipped with Airyscan detector, spectral (32-channel) PMT detector, 7 laser lines, wide range of objectives, everything needed for live imaging (t°, CO2, definite focus), fully motorized.
  2. Wide-field fluorescence microscope Zeiss Axio Observer Z1
    Equipped with excellent B/W and color cameras, LED or arc lamp illumination, wide range of filters, wide range of objectives, everything needed for live imaging (t°, CO2, definite focus), fully motorized.
    Latest software (ZEN blue) with many options, e.g. possibility to acquire large fields of view (tile scanning) or automate 96-well plate imaging.

Contact:

Facility manager Academic supervisor
optique003
Clément CHEVALIER
Clement.Chevalier@ulb.ac.be

Benoît VANHOLLEBEKE
Benoit.Vanhollebeke@ulb.ac.be