Electron microscopy

Equipment

  1. Transmission Electron Microscope. Tecnai 10 (FEI)
  2. Environmental Scanning Electron Microscope. Quanta 200 FEG (FEI)
  3. Room temperature-and Cryo-Ultramicrotomes. Leica EM UC6 and UC7
  4. Critical point dryer Leica EM CPD030.
  5. High Vacuum CoatingSystem Leica EM MED020.

Applications

A. Transmission A. Electron Microscopy (TEM)

  1. Ultra-structural analysis of ultra-thin sections of tissues and isolated cells.
  2. Particle analysis by negative staining of phages, viruses and other nanoparticles.
  3. DNA and RNA imaging by spreading and shadowing techniques.
  4. R oom temperature- and cryo-ultramicrotomy (cryo-sectioning)
  5. I mmuno-gold localisation on resin-embedded ultra-thin sections, or on cryo-sections by the Tokuyasu method.
  6. Sample preparation for all the above mentioned analyses.

B. Scanning Electron Microscopy (SEM)

  1. Analysis of conductive (metalized) and non conductive (environmental) biological samples
  2. Analysis of nanomaterials
  3. Sample preparation for SEM

C. Qualitative and quantitative image analyses.

D. Consultancy and training in EM.

Contact:

Facility manager Academic supervisor
optique003
Clément CHEVALIER
Clement.Chevalier@ulb.ac.be
optique003
David PEREZ-MORGA
David.Perez-Morga@ulb.ac.be
1

Electron microscopy